FLOW CYTOMETRY
CASE STUDY
Our Approach: Stabilise, Batch, Ship
Our Flow Cytometry team have designed a sample processing package that solves this problem.
The process involves:
» Stabilising sample before shipping
» Batch samples from one
» Decrease variability
Split Processing in Practice
At the sample collection site:
» Start with the Resolian Sample Processing Package Kits
» Follow the Lab Manual
» Freeze and Ship
What’s in the Sample Processing Kit
Resolian provide kits containing everything the sample collection site needs:
- Pre-measured reagents, ready to use on receipt
- Pre-made buffers, supplied by the manufacturer or Resolian
- All other consumables needed for collection and stabilisation
- A step-by-step lab manual, written for entry-level lab skills, so any trained analyst can run the process
Stability Data: Up to 47 Days Post-Sample
Freshly Lysed Blood represents traditional way of doing this. In the graph below we are showing up to 35 days post sample. The graph below shows data at 47 days with the error bars representing the replicate analysis with CV ranges between 9.4-6.2 and MdFI CV ranges between 2.0-4.7.
Designed for Any Lab-Trained Analyst
The package we have designed can be used by any lab-trained staff.
For example, an analyst. Shown below is an example of a method-naive analyst generally trained in lab procedures, compared to analyst primarily working on the project.
Beyond Phosphorylation: Receptor Occupancy Assays
The concept of split processing works for Receptor Occupancy Assay with unique order and stabilisation:
The Practical Difference
- Lower Batch Fees. We can now batch samples into larger batches without concerns over stability.
- Reduced Variability. Batching per subject reduces variability as the same reagent, instrument, day, and run will be used.
- Repeat Analysis. We have the luxury to repeat analysis if a batch fails.
- Automation. Batching allows us to use automation more regularly further reducing fees and improving precision.
- Multiple Centre Studies. We can handle studies conducted in multiple locations. Customs and shipping delays do not affect the samples and results.
- Low Logistics Costs. Samples can be shipped in fewer packages, reducing costs.
It Takes a Team, Working Fast, to Get This Right
Samples reach our lab within 15 minutes of arrival on site.
Our team works nights and weekends when studies demand it,
backed by courier and central lab relationships built over years, not weeks.
Every result passes through analysts, scientists, and a scientific director before it reaches you.
That’s the difference between a sample processing package and a partner.
